Journal: Science Advances
Article Title: No replication of direct neuronal activity–related (DIANA) fMRI in anesthetized mice
doi: 10.1126/sciadv.adl0999
Figure Lengend Snippet: ( A ) Gaussian reference functions for 5 × 5–voxel regions of the active contralateral thalamus (red) and S1BF (blue). Neuronal responses in the thalamus and S1 are expected to peak at ~15 and ~ 25 ms after the onset of whisker stimulus [figure 2 in the study by Toi et al. ], respectively. In three mice with significant BOLD responses also in the contralateral thalamus, a Gaussian reference function with a FWHM of ~15 ms peaked at 25 ms in the S1BF (blue) and 15 ms in the thalamus (red) was used to cross-correlate time courses of individual trials. In each animal, trials were selected, on the basis of a threshold of top 20, 50, and 80% cross-correlation values in each region. Two sets of selected trials (i.e., cortical and thalamic selection) for each threshold were obtained. ( B ) Averaged time courses of the S1BF and thalamus ROI obtained from the S1BF-selected trials. Blue, S1BF time course; red, thalamus. Error bars, SEM. ( C ) Cross-correlation map of one mouse (mouse #5). For the top 20% trials selected for the S1BF, voxel-wise cross-correlation values with Gaussian neural response functions peaking between 10 and 30 ms were calculated, and the highest cross-correlation values were mapped with a correlation amplitude threshold of 0.4 and a minimum of three contiguous voxels (see also fig. S4). ( D ) Averaged time courses of the S1BF and thalamus ROI obtained from the thalamus-selected trials. Blue, S1BF time course; red, thalamus. Artifactual peak intensity in the selected region was closely dependent on the selection threshold, while no obvious peak was observed in the unselected region. Error bars, SEM.
Article Snippet: The DIANA response function was modeled as a Gaussian window function in MATLAB, “gausswin,” with a peak position at 25 ms after the stimulus and the full width at half maximum (FWHM) of ~25 ms.
Techniques: Whisker Assay, Selection
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![( A ) Gaussian reference functions for 5 × 5–voxel regions of the active contralateral thalamus (red) and S1BF (blue). Neuronal responses in the thalamus and S1 are expected to peak at ~15 and ~ 25 ms after the onset of whisker stimulus [figure 2 in the study by Toi et al. ], respectively. In three mice with significant BOLD responses also in the contralateral thalamus, a Gaussian reference function with a FWHM of ~15 ms peaked at 25 ms in the S1BF (blue) and 15 ms in the thalamus (red) was used to cross-correlate time courses of individual trials. In each animal, trials were selected, on the basis of a threshold of top 20, 50, and 80% cross-correlation values in each region. Two sets of selected trials (i.e., cortical and thalamic selection) for each threshold were obtained. ( B ) Averaged time courses of the S1BF and thalamus ROI obtained from the S1BF-selected trials. Blue, S1BF time course; red, thalamus. Error bars, SEM. ( C ) Cross-correlation map of one mouse (mouse #5). For the top 20% trials selected for the S1BF, voxel-wise cross-correlation values with Gaussian neural response functions peaking between 10 and 30 ms were calculated, and the highest cross-correlation values were mapped with a correlation amplitude threshold of 0.4 and a minimum of three contiguous voxels (see also fig. S4). ( D ) Averaged time courses of the S1BF and thalamus ROI obtained from the thalamus-selected trials. Blue, S1BF time course; red, thalamus. Artifactual peak intensity in the selected region was closely dependent on the selection threshold, while no obvious peak was observed in the unselected region. Error bars, SEM.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1415/pmc10971415/pmc10971415__sciadv.adl0999-f5.jpg)
